Mold design is a critical engineering discipline in injection molding, particularly for high-performance thermoplastics like polyamide 66 (PA66) and its glass-filled variants. It encompasses the systematic planning of mold geometry, cooling systems, gating, venting, and ejection mechanisms to ensure part quality, production efficiency, and mold longevity. For materials such as PA66 with 25% glass fiber (GF25), designers must account for the abrasive nature of fibers, which necessitates the use of wear-resistant materials like hardened steels (e.g., H13) or surface treatments to mitigate erosion. Gate design—whether pinpoint, submarine, or hot runner—affects fiber orientation and weld lines, influencing mechanical properties like tensile strength and impact resistance. Cooling channels must be optimized to manage thermal conductivity and prevent warpage, as PA66 GF25 has a melting point around 260°C and recommended mold temperatures of 80–120°C. Shrinkage behavior, typically 0.2–0.5% in-flow and 0.5–0.8% cross-flow due to fiber reinforcement, requires precise dimensional allowances. Ejection systems should avoid part damage, leveraging stripper plates or air assists for complex geometries. Venting is essential to eliminate air traps and burns, often using micro-vents or porous inserts. Advanced simulation tools aid in predicting flow, cooling, and structural integrity, reducing trial cycles. In industries like automotive and aerospace, mold design must align with high-volume production needs and regulatory standards, emphasizing collaboration between material scientists and engineers to address challenges such as anisotropic behavior and thermal stability. Ultimately, a holistic approach integrating material properties, processing parameters, and end-use requirements is vital for achieving consistent performance and cost-effectiveness in molded components.